[expand title="What limit of detection can I expect with a 14C labeled protein in tissues?" tag="h2"]It depends upon the specific activity of the protein but typically you can expect an LOQ of 100-10 pg per gram of tissue.[/expand]
[expand title="How easy is it to 14C label the protein?" tag="h2"]Xceleron has two methods: the first is to chemically label by conjugating the protein with a 14C reagent such as 14CH3. This is similar technology to well established Iodine labelling. The advantage of this method is that it can be done very quickly and cost effectively. The disadvantage is that it does chemically modify the protein although this is usually acceptable for early screening studies.
The second method is to include 14C precursors such as a carbon source or amino acids into a fermentation vessel. Xceleron has access to facilities that do this type of labeling. The advantage is that the protein is not chemically altered, the 14C is incorporated within the structure. The disadvantage is that this takes longer.[/expand]
Microdosing studies: a consideration on analytical technology choice
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